| Solvent Black 3 Basic information |
| Product Name: | Solvent Black 3 |
| Synonyms: | LABOTEST-BB LT00451850;1H-Perimidine, 2,3-dihydro-2,2-dimethyl-6-((4-(phenylazo)-1-naphthyl)azo)-;Fast Black HB;Solvent Black 2;Sudan black b, high purity biological stain;SUDAN BLACK B (CERT);2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)naphthalen-1-yl]azo]-1H-perimidine;2,2-Dimethyl-6-{[4-(phenyldiazenyl)-1-naphthyl]-diazenyl}-2,3-dihydro-1H-perimidine |
| CAS: | 4197-25-5 |
| MF: | C29H24N6 |
| MW: | 456.54 |
| EINECS: | 224-087-1 |
| Product Categories: | Azo;stain;Solvent Dyestuff;Organics |
| Mol File: | 4197-25-5.mol |
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| Solvent Black 3 Chemical Properties |
| Melting point | 120-124 °C(lit.) |
| Boiling point | 552.68°C (rough estimate) |
| density | 1.4899 (rough estimate) |
| refractive index | 1.4570 (estimate) |
| storage temp. | Store at RT. |
| solubility | Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol |
| Colour Index | 26150 |
| form | Powder/Solid |
| pka | 2.94±0.40(Predicted) |
| color | Very dark brown to black |
| Water Solubility | Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water. |
| λmax | 598 nm, 415 nm |
| Merck | 13,8970 |
| BRN | 723248 |
| Stability: | Light Sensitive |
| Biological Applications | Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening |
| CAS DataBase Reference | 4197-25-5(CAS DataBase Reference) |
| EPA Substance Registry System | C.I. Solvent Black 3 (4197-25-5) |
| Safety Information |
| Hazard Codes | F,Xi |
| Risk Statements | 11-36/37/38 |
| Safety Statements | 24/25-16-37/39-26 |
| WGK Germany | 3 |
| RTECS | SD4431500 |
| TSCA | Yes |
| HazardClass | IRRITANT |
| HS Code | 32041900 |
| Toxicity | LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918 |
| Provider | Language |
|---|---|
| C.I. 26150 | English |
| SigmaAldrich | English |
| ACROS | English |
| Solvent Black 3 Usage And Synthesis |
| Chemical Properties | VERY DARK BROWN TO BLACK FINE CRYSTALLINE POWDER |
| Uses | Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing. |
| Preparation | aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling. |
| Definition | ChEBI: A member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carring a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in rozen sections and for staining of protein bound lipids in paraffin sections. |
| Safety Profile | A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx. |
| Properties and Applications | Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper. StandardLight FastnessHeat-resistant(℃)waterSodium Carbonate(5%)Hydrochloric acid(5%)Melting pointStableISOGeneral126100InsolubleNo changeNo change |
| Solvent Black 3 Preparation Products And Raw materials |
| Raw materials | 1-Naphthylamine–>Aniline–>1-Naphthylamine–>2,2-dimethyl-2,3-dihydro-1H-perimidine |