Rhodamine B

Rhodamine B Basic information
Product Name:Rhodamine B
Synonyms:9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride;(9-(O-CARBOXYPHENYL)-6-(DIETHYL-AMINO)-3H-XANTHENE-3-YLIDENE)DIETHYL AMMONIUM CHLORIDE;ACID RHODAMINE B;LABOTEST-BB LT00772274;BRILLIANT RHODAMINE B;BRILLIANT PINK;BRILLIANT PINK B;CI NO 5170
CAS:81-88-9
MF:C28H31ClN2O3
MW:479.01
EINECS:201-383-9
Product Categories:Analytical Chemistry;Ion Association;Steroids, Terpenes, Lipids, Bile acidsDerivatization Reagents;TLC Reagents, N-R;TLC ReagentsDerivatization Reagents TLC;Derivatization Reagents TLC;TLC Visualization Reagents (alphabetic sort);TLC Visualization Reagents (by application);Organics;Dyes and Pigments
Mol File:81-88-9.mol
Rhodamine B Structure
Rhodamine B Chemical Properties
Melting point 210-211 (dec.)(lit.)
density 0.79 g/mL at 20 °C
refractive index 1.6500 (estimate)
Fp 12 °C
storage temp. room temp
solubility H2O: soluble1mg/mL
Colour Index 45170
form Solid
color Green
PH3-4 (10g/l, H2O, 20℃)
Water Solubility SOLUBLE
Merck 14,8183
BRN 4119648
Stability:Stable. Incompatible with strong oxidizing agents.
LogP1.9-2
CAS DataBase Reference81-88-9(CAS DataBase Reference)
NIST Chemistry ReferenceRhodamine b(81-88-9)
IARC3 (Vol. 16, Sup 7) 1987
EPA Substance Registry SystemRhodamine B (81-88-9)
Safety Information
Hazard Codes Xn,Xi,F
Risk Statements 22-41-68-67-36-11-40-20/21/22-52/53
Safety Statements 7-16-24/25-26-36/37/39-39-36-22
RIDADR UN 3077 9/PG 3
WGK Germany 3
RTECS BP3675000
TSCA Yes
HS Code 32041300
Hazardous Substances Data81-88-9(Hazardous Substances Data)
ToxicityLD50 i.v. in rats: 89.5 mg/kg (Webb)
MSDS Information
ProviderLanguage
Rhodamine BEnglish
SigmaAldrichEnglish
ACROSEnglish
ALFAEnglish
Rhodamine B Usage And Synthesis
Chemical PropertiesC.I. Food red 15 is a green crystalline or redviolet powdered solid
Chemical Propertiesred/brown or green crystals
UsesAs a dye, especially for paper; as a reagent for antimony, bismuth, cobalt, niobium, gold, manganese, mercury, molybdenum, tantalum, thallium, tungsten; as biological stain. Provisionally listed for use in drugs and cosmetics.
UsesA useful fluorochrome for histology, FRET and mitochondrial probe.
UsesRhodamine B is used in biological studies for an anti-reactive oxygen species/hepatic fibrosis drug delivery system based on salvianolic acid B loaded mesoporous silica nanoparticles. Dyes and metabolites, Environmental Testing.
DefinitionChEBI: An organic chloride salt having N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium as the counterion. An amphoteric dye commonly used as a fluorochrome.
General DescriptionGreen crystals or reddish-violet powder. Used as a dye, especially for paper, as a metal chelating reagent, and in drugs and cosmetics.
Air & Water ReactionsVery soluble in water. Solution is bluish-red. Dilute solutions are strongly fluorescent.
Reactivity ProfileAcidic organic/inorganic salts, such as Rhodamine B, are generally soluble in water. The resulting solutions contain moderate concentrations of hydrogen ions and have pH’s of less than 7.0. They react as acids to neutralize bases. These neutralizations generate heat, but less or far less than is generated by neutralization of inorganic acids, inorganic oxoacids, and carboxylic acid. They usually do not react as either oxidizing agents or reducing agents but such behavior is not impossible. Many of these compounds catalyze organic reactions.
HazardQuestionable carcinogen.
Potential ExposureIt is used as a color additive in drugs, foods, cosmetics, and fabric dyes. It is also used as a tracing agent in water pollution studies. May be used as an agricultural chemical
ShippingUN3143 Dyes, solid, toxic, n.o.s. or Dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.
Purification MethodsMajor impurities are partially dealkylated compounds not removed by recrystallisation.Purify the dye by chromatography, using ethyl acetate/isopropanol/ammonia (conc)(9:7:4, RF 0.75 on Kieselgel G). It has also been crystallised from a concentrated solution in MeOH by slow addition of dry diethyl ether; or from EtOH containing a drop of conc HCl by slow addition of ten volumes of dry diethyl ether. The solid is washed with ether and air dried. The dried material has also been extracted with *benzene to remove oil-soluble material prior to recrystallisation. Store it in the dark. [Beilstein 18 II 486, 18 III/IV 8246, 19/8 V 669.]
Properties and ApplicationsTEST ITEMSSPECIFICATIONAPPEARANCEGREEN GLITTERY CRYSTALSHADE (COMPARE TO STANDARD)CLOSEWATER SOLUBILITY (60°C)8 g/L minWATER INSOLUBLE0.5% maxTINTING STRENGTH500-505 %
IncompatibilitiesDust may form explosive mixture with air. Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides, strong reducing agents
Rhodamine B Preparation Products And Raw materials
Raw materialsPhthalic anhydride–>Sulfonated castor oil–>3-Diethylaminophenol–>Yellow dextrin–>FLUORESCEIN CHLORIDE–>3-Diethylaminophenol–>Diethylamine–>Phthalic anhydride
Preparation ProductsFast Pink lake–>Spirit Light Fast Fire Red B–>BUTYL RHODAMINE B–>2-(4-Diethylamino-2-hydroxybenzoyl)benzoic acid

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