| Basic Red 5 Basic information |
| Product Name: | Basic Red 5 |
| Synonyms: | AMinodiMethylaMinotoluaMinozine;Neutral Red, high purity biological stain, pure 25GR;NEUTRAL RED (C.I. 50040) INDICATOR AND F;NEUTRAL RED (C.I. 50040) FOR MICROSCOPY;Neutral Red, Reagent, ACS;Neutral Red, high purity biological stain;Neutral Red, indicator (pH 6.8 – 8.0);2,8-PhenazinediaMine,N8,N8,3-triMethyl-, hydrochloride (1:1) |
| CAS: | 553-24-2 |
| MF: | C15H17ClN4 |
| MW: | 288.78 |
| EINECS: | 209-035-8 |
| Product Categories: | Phenazine;Azine;Miscellaneous;marker;Organics;Analytical Chemistry;Indicator (pH);pH Indicators;Stains and Dyes |
| Mol File: | 553-24-2.mol |
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| Basic Red 5 Chemical Properties |
| Melting point | 290 °C (dec.) (lit.) |
| Boiling point | 441.31°C (rough estimate) |
| density | 1.1590 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | Store at +5°C to +30°C. |
| solubility | H2O: soluble10mg/mL |
| form | Solid |
| Colour Index | 50040 |
| pka | 6.7, 7.4(at 25℃) |
| color | Very dark green |
| PH Range | 6.8(red)-8(yellow) |
| PH | 3.1 (10g/l, H2O, 20℃) |
| Odor | Odorless |
| Water Solubility | 50 g/L |
| λmax | 540nm, 533nm, 544nm, 529nm, 454nm |
| Merck | 14,6488 |
| BRN | 3918740 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| Biological Applications | Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases |
| Major Application | Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases |
| CAS DataBase Reference | 553-24-2(CAS DataBase Reference) |
| EPA Substance Registry System | Neutral Red (553-24-2) |
| Safety Information |
| Hazard Codes | Xn,Xi |
| Risk Statements | 68-36/37/38 |
| Safety Statements | 36/37-37/39-36-26-24/25 |
| WGK Germany | 3 |
| RTECS | SG1400000 |
| TSCA | Yes |
| HS Code | 32041300 |
| Toxicity | LD50 intraperitoneal in mouse: 432mg/kg |
| Provider | Language |
|---|---|
| Neutral Red | English |
| Basic Red 5 Usage And Synthesis |
| Chemical Properties | dark green powder |
| Uses | Can be used as a vital stain, to stain living cells. |
| Uses | Neutral Red is a common histology stain used to determine cell viability.Dyes and metabolites. |
| Uses | Neutral Red is used as indicator for nitrogen determination; pH indicator. |
| Application | Transition of pH Indicator: pH 6.8 – 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it. |
| Preparation | (a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture. |
| Definition | ChEBI: A hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. |
| General Description | Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl. |
| Biochem/physiol Actions | Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes. |
| Purification Methods | In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.] |
| Properties and Applications | red light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation. |
| Basic Red 5 Preparation Products And Raw materials |