Basic Red 5

Basic Red 5 Basic information
Product Name:Basic Red 5
Synonyms:AMinodiMethylaMinotoluaMinozine;Neutral Red, high purity biological stain, pure 25GR;NEUTRAL RED (C.I. 50040) INDICATOR AND F;NEUTRAL RED (C.I. 50040) FOR MICROSCOPY;Neutral Red, Reagent, ACS;Neutral Red, high purity biological stain;Neutral Red, indicator (pH 6.8 – 8.0);2,8-PhenazinediaMine,N8,N8,3-triMethyl-, hydrochloride (1:1)
CAS:553-24-2
MF:C15H17ClN4
MW:288.78
EINECS:209-035-8
Product Categories:Phenazine;Azine;Miscellaneous;marker;Organics;Analytical Chemistry;Indicator (pH);pH Indicators;Stains and Dyes
Mol File:553-24-2.mol
Basic Red 5 Structure
Basic Red 5 Chemical Properties
Melting point 290 °C (dec.) (lit.)
Boiling point 441.31°C (rough estimate)
density 1.1590 (rough estimate)
refractive index 1.6110 (estimate)
storage temp. Store at +5°C to +30°C.
solubility H2O: soluble10mg/mL
form Solid
Colour Index 50040
pka6.7, 7.4(at 25℃)
color Very dark green
PH Range6.8(red)-8(yellow)
PH3.1 (10g/l, H2O, 20℃)
OdorOdorless
Water Solubility 50 g/L
λmax540nm, 533nm, 544nm, 529nm, 454nm
Merck 14,6488
BRN 3918740
Stability:Stable. Incompatible with strong oxidizing agents.
Biological ApplicationsDetecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases
Major ApplicationFuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases
CAS DataBase Reference553-24-2(CAS DataBase Reference)
EPA Substance Registry SystemNeutral Red (553-24-2)
Safety Information
Hazard Codes Xn,Xi
Risk Statements 68-36/37/38
Safety Statements 36/37-37/39-36-26-24/25
WGK Germany 3
RTECS SG1400000
TSCA Yes
HS Code 32041300
ToxicityLD50 intraperitoneal in mouse: 432mg/kg
MSDS Information
ProviderLanguage
Neutral RedEnglish
Basic Red 5 Usage And Synthesis
Chemical Propertiesdark green powder
UsesCan be used as a vital stain, to stain living cells.
UsesNeutral Red is a common histology stain used to determine cell viability.Dyes and metabolites.
UsesNeutral Red is used as indicator for nitrogen determination; pH indicator.
ApplicationTransition of pH Indicator: pH 6.8 – 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.
Preparation(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.
DefinitionChEBI: A hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0.
General DescriptionNeutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.
Biochem/physiol ActionsNeutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.
Purification MethodsIn aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]
Properties and Applicationsred light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation.
Basic Red 5 Preparation Products And Raw materials
Raw materials2,4-Diaminotoluene–>N,N-DIMETHYL-4-NITROSOANILINE–>N,N-Dimethyl-1,4-phenylenediamine

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